Plasmid

Part:BBa_K4184004:Design

Designed by: Fabienne Spang   Group: iGEM22_TU_Braunschweig   (2022-10-08)


NpuN_NTEV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Creating the whole thing was a little bit difficult


Source

Gramespacher JA, Stevens AJ, Nguyen DP, Chin JW, Muir TW. Intein Zymogens: Conditional Assembly and Splicing of Split Inteins via Targeted Proteolysis. J Am Chem Soc. 2017 Jun 21;139(24):8074-8077. doi: 10.1021/jacs.7b02618. Epub 2017 Jun 7. PMID: 28562027; PMCID: PMC5533455.


References